Clinical Services

 Newcastle University

  Bacterial Cell Biology

  School of Biology

Erasmus Medical Center

https://www.erasmusmc.nl/47439/245704/4601462/4570071/Fungalinfections?lang=en

 

Wellcome Trust Centre for Global Health Research

https://www.bsms.ac.uk/index.aspx

 

Stockholm University

http://www.su.se/english/profiles/gebre-1.182447

 

Maynooth University, Department of Biology Maynooth, Ireland

Professor Sean Doyle

https://www.maynoothuniversity.ie/people/sean-doyle

 

Centers for Disease Control and Prevention

cdc

https://www.cdc.gov/fungal/diseases/mycetoma/index.html

 https://www.cdc.gov/fungal/diseases/mycetoma/health-professionals.html

Association for Aid and Relief, Japan

(AAR Japan) http://www.aarjapan.gr.jp/english/activity/sudannorth/

AISPO Italy http://aispo.org/en/projects/ http://aispo.org/en/progetti/sudan-support-to-medical-education-of-public-institutions-in-kassala-state/

 

University of Applied Sciences and Arts, Hochschule Hannover, Germany

http://f3.hs-hannover.de/personen/lehrende/fortwengel-gerhard-dr-prof/werdegang/index.html

 

Salvatore Rubino  Professor of Microbiology Editor in Chief Journal of Infection in Developing Countries

Dipartimento di Scienze Biomediche, Viale San Petro 43B 07100 Sassari (Italy)

 

mrc commdeve

 

 

Outreach Villages Based Clinical Services

Many patients tend to present late with advanced disease where amputation of the affected part may be the only possible treatment. Most of these patients are from under-developed communities, and hence one of the important functions of the MRC is to reach these patients in their own villages and to provide them with free medical and surgical treatment as well as health educations

To execute this, the centre had conducted many field trips to endemic areas where health education sessions were organised, out-patient clinics were conducted, patients were treated using mobile surgical units and team free of charge. Some patients were referred to MRC or specialisedcentres for further management.

Continuing Professional Development

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Interesting Cases

Rare presentation of actinomycetoma

Case Report
A.H. Fahal and H. E. Sheik
Mycetoma Research Centre,
University of Khartoum,
Khartoum-Sudan

The patients; is a 36 year-old gentleman, farmer from Western Sudan. He presented to the Mycetoma Clinic at Soba University Hospital, Khartoum, Sudan with massive swelling of the left side of the back extending down to the gluteal region. The condition stared 18 years with a small painless swelling in the left lower part of his back at a site of trauma following a fall from a tree. It then gradually regressed in size. Two year later the swelling gradually stared to increase in size, sinuses discharging yellow grains then developed. He had no past history of bilharziasis or haematemesis. His social, family or drug history is not contributory to his condition.

On examination he looks unwell and slightly pale. Head and neck, chest and cardio-vascular, central nervous system examination are within normal. He has gynaecomastia of the left breast, which is tender and firm in consistency. Abdominal examination revealed multiple distended veins extending from the left hypochondrial region to the left iliac fossa. The spleen is grossly enlarged otherwise normal clinical findings.

Local examination of the back revealed huge mass involving the whole left side of back extending to the chest wall and down to the gluteal region. The mass was painless, firm in consistency with areas of hypopigmentation. The skin was tethered to the deep structures with multiple healed sinuses.

His full blood count and ESR are within normal. Abdominal ultrasound showed an enlarged spleen with normal texture, normal splenic vein and evidence of bilharzial periportal fibrosis. Intravenous urography showed normal function of both kidneys, the left ureter was deviated medically and erosion of the left 12 rib and the first five lumber vertebrae.  Abdominal CT scan showed a huge mass at the back pushing the left kidney and ureter medical with erosion of the first five lumber vertebrae. An incisional biopsy was taken under local anesthesia and it confirmed the diagnosis of actinomycetoma due to Streptomyces somaliensis. MRI examination showed huge soft tissue mass.

The patient was stared on streptomycin sulphate in a dose of 14 mg/kg daily for one month then on alternate days and diaminodiphenyl sulphone (dapsone) in a dose of 1.5 mg/kg twice daily and Co-trimoxazole in a dose of 14 mg/kg twice daily. His portal hypertension was treated conservatively.  At two months follow up he showed dramatic clinical improvement the mass had regressed in size, sinuses had healed and the skin became less tethered to the underline tissue.

 

mrc outpatient

 

The MRC has an out-patient clinic, conducted every Monday morning where patients are seen regularly for treatment and follow up. Health education sessions organized by health workers are conducted regularly. Patients’ counseling is done by the MRC Social workers. The clinic accepts patients from different part of the country and the region as well.

 

Continuing Professional Development

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Mycetoma Pathology

Prof. A.M. EL Hassan. MRCP, MRCPath, PhD, DCP
Emeritus Professor of Pathology.
Mycetoma Research Group.
Institute of Endemic Diseases.
University of Khartoum.
E.mail:

 

Mycetoma (maduromycosis) is a chronic granulomatous subcutaneous infection caused by actinomycetes (actinomycetoma) or by true fungi (eumycetoma). Clinically the disease is characterized by swelling, deformity and sinuses in the affected part (Fig.1). Another characteristic feature of mycetoma is the formation of aggregates of the organism (grains) in the tissues, which are visible to the naked eye and are discharged through sinuses in the skin (Fig. 2). The grains vary in colour, size and consistency depending on the causative agent. These features are helpful in making a tentative diagnosis of the causative organism. A definitive identification of the agent is established by the histological examination of the grains, by culture or by serologic techniques.  Both actinomycetoma and eumycetoma prevail in the Sudan. Eumycetoma due to Madurella mycetomatis is the commonest type. The actinomycetoma is commonly caused by Streptomyces somaliensis, Actinomadura madurae and Actinomadura pelletierii.

This, article describes the pathology of the commonest types of mycetomata in the Sudan in surgical material referred to the Pathology Department, Faculty of Medicine, University of Khartoum.

1.  M.  mycotomatis

It is the most common cause of eumycetoma in the Sudan. In clinical material, the grains in the tissue are black and numerous (Fig.3). In stained sections the grain is rounded, oval or trilobed. It has a more compact cortex, which is dark brown in colour due to pigment produced by the organism and has a lighter medulla. In some grains the division into cortex and medulla is not evident. The grain filaments are usually embedded in a hard brown cement matrix.

Two main morphological types of grains are identified. The filamentous, which is the commonest type, consists of brown septate and branched hyphae that may be slightly more swollen towards the periphery (Fig. 4). In the cortex, the filaments are arranged radially while in the medulla they tend to run multi-directionally. Round or oval cells, 7-15 um in diameter, are seen, particularly in the periphery.

The second type of grain is the vesicular one (Fig. 5). It is less common than the filamentous and is composed of unusually large cells that look like vesicles. Both types of the grain can be found in the same lesion. Grains that are partially vesicular and partially filamentous are not uncommonly seen.

The surface of the grain may be scalloped giving the periphery a hob-nail appearance and a brightly eosinophilic layer of fibrin–like material sometimes covers the grain (Fig. 6). This layer is of host origin, contains fibrin, immunoglobulins and complement. A similar material is seen around parasites as Hoepple phenomenon. Occasionally a thin layer of a haematoxophilic granular material is found on the surface of the grain. This material is Feulgen positive and is derived from the nuclei of disintegrating inflammatory cells surrounding the grains. The inflammatory cellular reaction around the grain is variable. There are three types of tissue reaction. In type I, there is a zone of neutrophils in the vicinity of the grain (Figs. 7,8). These are sometimes found within the grain substance causing its disintegration. Some histocytes may also be seen among the neutrophils but they are more numerous outside the neutrophil zone. Some of the histocytes have a foamy cytoplasm and give a positive reaction for fat and stain positive for CD 68 antigen (Figs.9,10). Capillaries, which are sometimes abundant, surround the neutrophil/ histiocyte zone and they are occasionally, surrounded by a layer of fibrin. Lymphocytes, plasma cells, fibroblasts and some macrophages are usually seen in this vascular zone. The lymphocytes and plasma cells increase in number towards the investing fibrous tissue in the periphery of the lesion.

In type II reaction, the neutrophils largely disappear and are replaced by histocytes and multinucleated giant cells (Fig 11). Some of the latter contain fragments of grain or pigmented cement substance without any hyphae. The macrophages may contain a black pigment derived from the grain (Fig. 12). At this stage the grain itself is usually small and fragmented. This type of histocytes/giant cell reaction follows on the earlier neutrophil response, which causes fragmentation of the grain.

In the third type of reaction (Type III), the grain material has largely or completely disappeared leaving a compact epithelioid granuloma with or without langerhans giant cells (Figs.13, 14). This however, is an uncommon reaction but represents spontaneous regression in some grains.

The three types of tissue reaction may be found in the same lesion. Viable grains are nearly always present in biopsy material. It is not known if spontaneous regression of all the grain ever occurs in mycetoma. The unique feature of M. mycetomatis is the formation of a capsule around the lesion. The lesion grows by expansile growth in the tissue plains. In the bones there is usually no capsule formation, the organism usually forming cavities that are filled with the grains. This gives the bone support and may explain the rarity of pathological fractures in mycetoma.

Ultrastructurally the hyphal elements are spherical to elongated and are embedded in grain matrix (Fig. 15). The hyphae are septate, the cytoplasm may be densely ribosomal or disorganized. Some hyphae appear empty, being devoid of cytoplasm. Cytoplasmic organelles such as nuclei and mitochondria are not usually seen. Intra–hyphal re-growth is sometimes seen. The hyphal wall is often markedly thickened. This feature may be involved in the transformation of the fungus to the pathogenic state.

The pigmented substance surrounding the hyphae consists of amorphous electron-dense material and vesicles. The nature of the pigment is not known with certainty. Histologically it resembles melanin and it may be a fungus product. Sclerosis and melanization of the host tissue is, in some manner, responsible for the formation of the cement substance.

Ultrastructural studies of the host reaction show neutrophils adherent to the grain (Figs.16,17,18). The cytoplasm of the neutrophil is stretched over the grain and the neutrophils granules are concentrated in the part of the cytoplasm adjacent to the grain. This is an immune adherence, which is mediated by immunoglobulins and is an example of antibody dependent cell mediated cytotoxicity. Immunoglobulin and complement can be demonstrated in the grain (Figs.19, 20).

Bones are frequently involved in advanced mycetoma of the soft tissue (Figs. 21,22,23). Occasionally primary bone mycetoma in the absence of soft tissue involvement is seen. M. mycetomatis produces lytic lesions which are  large in size, few in number and have well defined margins; this is well seen radiologically.

   

2. Streptomyces somaliensis:

The grains are yellow in colour and hard in consistency. During surgery it may be difficult to distinguish the grains from fat which makes radical excision of the lesion difficult. This is especially so since the lesion is not encapsulated. In sections the grain is rounded to oval, dense and homogenous. Characteristically marks of the microtome knife are seen in the grain in the form of parallel cracks. The grain stains a light purple or pink colour in haematoxylin and eosin stained sections (Fig 7). The grain varies in size from 30 to 200 um. Hyphal elements embedded in cement can be visualized by Gram stain.

The grain is surrounded by an intense neutrophil polymorphonuclear leucocyte infiltrate (Type 1). Outside this zone, there is a vascular layer containing macrophage, lymphocytes, plasma cells and giant cells. The giant cells usually contain fragments of the grain. Some macrophages have a foamy cytoplasm. It looks as though the fragmentation of the grain induced by neutrophils is less severe than in M. mycetomatis. This may be due to the more compact and hard grains of S. somaliensis. Small grains surrounded by macrophages and giant cells (Type 11) are occasionally seen but pure epithelioid granuloma (Type 111) apparently does not occur. Giant cells containing viable actinomycetes are believed to aid the spread of the organism in the tissue and to the regional lymph nodes. Despite the invasive nature of S. somaliensis and other actinomycetes, tendons and nerves are resistant to invasion.

Ultrastrcturally the grain consists of heterogenous and amorphous matrix arranged in an irregular and reticulate structure surrounding electron lucent areas between 1 and 5 um. In some of these spaces bacterial filament are found. The organisms are usually unicellular and coccoid and the cell wall is electron dense.

   

3. Actinomadura pelletierii:

The grains in clinical material are tiny and red in colour (Fig. 24). In section the grain is rounded, oval or semilunar. It stains a purple colour and compact hyphae give it the appearance of “Iron filings”. The periphery of the grain has a narrow deeply eosinophilic band. The grain is usually surrounded by a zone of neutrophils which causes fragmentation of the grain. The other layers are similar to those seen in S. somaliensis but the giant cells are less conspicuous.

The ultrastructure of the grain is quite distinctive. The hyphae are septate, compact without cement substance and under low magnification the hyphae have a starry sky appearance because of the vacuoles in the hyphae. These are probably fixation artifacts. Neutrophils usually adhere to the grain and degranulate. Grain material is phagocytosed by the neutrophils and destroyed.

4. Actinomadura madurae:

Macroscopically the grains are yellow or white. They are difficult to distinguish from the surrounding fat. Histologically the large grains have a characteristic variegated pattern (Fig. 25). The periphery of the grain is dense, homogenous and deep purple while the centre is less dense or even appears hollow. Not infrequently the grain fragments into geometric fragments. The periphery shows a brightly eosinophilic material forming clubs. This, material contains immunoglobulins. Smaller grains are more homogeneous and are difficult to distinguish from A. pelletierii.

However, even the small grains of A. madurae have a more deeply stained purple fringe, which is not seen in A. pelletierii. The inflammatory reaction is similar to that of A. pelletierii.

5.  Vascular changes in mycetoma

The arteries and veins in the mycetoma lesion show hypertrophy of the muscles (Fig. 26). The lumen is narrowed but is not occluded completely. Grain fragments are occasionally seen within the blood vessels. These may explain the rare haematogenous spread to distant sites.

6. Cytopathology of mycetoma

Fine needle aspiration cytology of mycetoma was described. Mycetoma has  distinct cytological features characterized by the presence of polymorphous inflammatory cells consisting of neutrophils, lymphocytes, plasma cells, histocytes and foreign body giant cells. The causative organism is identified in most of the cases, surrounded or/ and infiltrated by neutrophils. M. mycetomatis grains are rounded or oval in shape. In Giemsa stained smears, they appear black with green tinge of colour and brownish in H&E.  Two types of M. mycetomatis grains could be identified in cytological smears, the solid granular one, which is the commonest and the vesicular. The septate hyphae are not identified in the first type as they are embedded in a hard brown cement matrix. The vesicular type consists of swollen fungal cells, seen as vesicles.

Actinomycetes grains are homogeneously eosinophilic in H&E (Fig. 27). In Geimsa stained smears the grain appears homogeneously blue in the center while in the periphery it consists of fine granules and radiating pink filaments.

The A. pelletieri grain is more eosinophilic in H&E as compared to S. somaliensis and it is semilunar in shape as seen in histology. To distinguish between actinomycetoma and eumycetoma, fine needle aspiration cytology was found to be as accurate as histopathology when grains are present. The cytological features of mycetoma correlates well with the histologic one.

The technique is simple, quick and cheap. It can be introduced for routine diagnosis of mycetoma and for epidemioloical surveys and for sample collection for culture.

7.  Bony changes in mycetoma:

 Bones are frequently involved in advanced mycetoma of the soft tissue. Occasionally primary bone mycetoma in the absence of soft tissue involvement is seen. M. mycetomatis produces lytic lesions which are  large in size, few in number and have well defined margins.

Actinomycetoma destroys bone and also evokes new bone formation. The cavities produced are usually smaller in size, numerous and have no definite margins.  A periosteal reaction with new bone formation and sun-ray appearance may be seen in radiographs. This features along with Codaman’s triangle simulates the radiographic changes in osteogenic sarcoma.

8.  Lymph nodes in mycetoma:

Lymph nodes draining a mycetoma focus are frequently enlarged. Most of the node shows only reactive hyperplasia with an intense plasma cell infiltration. The plasma cells often contain Russell bodies. These changes may be due to antigens reaching the node from grains at the primary site. Secondary bacterial infection of the mycetoma lesion may also be a factor. Occasionally the organism metastasizes to the nodes causing lymphadenitis with sinuses discharging grains (Fig. 28). This is more frequent with actinomycetoma than with eumycetoma. Not infrequently haemosiderin deposits are found in the nodes even in the absence of grains. The haemosiderin is derived from the granulation tissue in the peripheral lesion draining into the lymph nodes. Melanin is also found in the node and may be confused with the pigment of M. mycetomatis.

Further reading:

1.      EL Hassan AM, Fahal AH, EL Hag IA, Khalil EAG, The pathology of mycetoma: Light microscopic and ultrastructural features. Sudan Medical Jorunal. 1994; 32: 23-45.  

2.      Fahal AH, EL Toum EA, EL Hassan AM, Gumaa SA, Mahgoub ES, A preliminary study on the ultrastructure of Actinomadura pelletierii and its host tissue reaction. Journal of Medical and veterinary Mycology 1994; 32: 343-348.

3.      Mahgoub ES and Murray TG.  Mycetoma William Heineman, Medical Books, London.

4.      Fahal AH, EL Toum EA, EL Hassan AM, Gumaa SA, Mahgoub ES, Host tissue reaction to Madurella mycetomatis: New classification. Journal of Medical and veterinary Mycology. 1995; 33: 15-17.  

5.      EL Hag IA, Fahal AH, Khalil EAG, Fine needle aspiration cytology of mycetoma.   Acta Cytologica. 1996; 40(3): 461-464.  

6.      Fahal AH, EL Hag IA, Gadir AFA, EL Lider AR, EL Hassan AM, The blood supply and vasculature in mycetoma. Journal of Medical and veterinary Mycology. 1997;  35:101-106.