Morphological Description:
The minimum growth temperature for this fungus was 15°C, and the maximum was above 40°C, with an optimum at 36 to 37°C. The colony diameter reached only 1 to 2 mm when grown at 20°C. The isolate decomposed casein in the first week hydrolyzed Starch and Gelatin. Urease test showed to be strongly positive within 3 days.
Molecular Identification:
ITS sequencing is recommended.
In vitro antifungal activity
This fungus was found to be highly susceptible to the azoles itraconazole, ketoconazole, posaconazole, and voriconazole. Low MICs were also found for amphotericin B. The only azole for which a high MIC was found (128 μg/ml) was fluconazole. The fungus was inhibited by caspofungin at 8 μg/ml, but not by 5-flucytosine (MIC > 64 μg/ml).
References:
Mhmoud NA, Ahmed SA, Fahal AH, de Hoog GS, Gerrits van den Ende AH, van de Sande WW. Pleurostomophora ochracea, a novel agent of human eumycetoma with yellow grains. J Clin Microbiol. 2012;50(9):2987-2994.